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Effects of Fx on <t>TM3</t> cell viability in response to BPA exposure. (a) CCK-8 assay of TM3 cells treated with increasing concentrations of BPA; (b) effects of Fx alone on TM3 cell viability; (c) Fx and C34 attenuate BPA (50 μM)-induced cytotoxicity in TM3 cells. Data presentation and statistical analysis are as described for Figure 1 (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; DMSO, dimethyl sulfoxide.
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Effects of Fx on <t>TM3</t> cell viability in response to BPA exposure. (a) CCK-8 assay of TM3 cells treated with increasing concentrations of BPA; (b) effects of Fx alone on TM3 cell viability; (c) Fx and C34 attenuate BPA (50 μM)-induced cytotoxicity in TM3 cells. Data presentation and statistical analysis are as described for Figure 1 (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; DMSO, dimethyl sulfoxide.
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Effects of Fx on <t>TM3</t> cell viability in response to BPA exposure. (a) CCK-8 assay of TM3 cells treated with increasing concentrations of BPA; (b) effects of Fx alone on TM3 cell viability; (c) Fx and C34 attenuate BPA (50 μM)-induced cytotoxicity in TM3 cells. Data presentation and statistical analysis are as described for Figure 1 (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; DMSO, dimethyl sulfoxide.
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Effects of Fx on TM3 cell viability in response to BPA exposure. (a) CCK-8 assay of TM3 cells treated with increasing concentrations of BPA; (b) effects of Fx alone on TM3 cell viability; (c) Fx and C34 attenuate BPA (50 μM)-induced cytotoxicity in TM3 cells. Data presentation and statistical analysis are as described for Figure 1 (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; DMSO, dimethyl sulfoxide.

Journal: Balkan Medical Journal

Article Title: Fucoxanthin Attenuates Bisphenol A-Induced Testicular Injury via NF-κB-Mediated Pyroptosis Inhibition

doi: 10.4274/balkanmedj.galenos.2026.2025-11-268

Figure Lengend Snippet: Effects of Fx on TM3 cell viability in response to BPA exposure. (a) CCK-8 assay of TM3 cells treated with increasing concentrations of BPA; (b) effects of Fx alone on TM3 cell viability; (c) Fx and C34 attenuate BPA (50 μM)-induced cytotoxicity in TM3 cells. Data presentation and statistical analysis are as described for Figure 1 (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; DMSO, dimethyl sulfoxide.

Article Snippet: The TM3 Leydig cell line (CL0234), TM3 Cell Complete Medium (CM-0234), and DMEM/F12 (PM150312) were provided by Procell Life Science & Technology (Wuhan, China).

Techniques: CCK-8 Assay

Effects of Fx on the pyroptosis signaling pathway in vitro . (a, b) The mRNA levels of Casp1 and Gsdmd ; (c) Western blotting analysis of the expression of Caspase-1, Caspase-1 p20, GSDMD, and N-GSDMD; (d-f) the quantification of the Western blotting analysis; (g, h) IL-1β and IL-18 concentrations in TM3 cell supernatant. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; GSDMD, gasdermin D; IL-1β, interleukin-1β; IL-18, interleukin-18.

Journal: Balkan Medical Journal

Article Title: Fucoxanthin Attenuates Bisphenol A-Induced Testicular Injury via NF-κB-Mediated Pyroptosis Inhibition

doi: 10.4274/balkanmedj.galenos.2026.2025-11-268

Figure Lengend Snippet: Effects of Fx on the pyroptosis signaling pathway in vitro . (a, b) The mRNA levels of Casp1 and Gsdmd ; (c) Western blotting analysis of the expression of Caspase-1, Caspase-1 p20, GSDMD, and N-GSDMD; (d-f) the quantification of the Western blotting analysis; (g, h) IL-1β and IL-18 concentrations in TM3 cell supernatant. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; GSDMD, gasdermin D; IL-1β, interleukin-1β; IL-18, interleukin-18.

Article Snippet: The TM3 Leydig cell line (CL0234), TM3 Cell Complete Medium (CM-0234), and DMEM/F12 (PM150312) were provided by Procell Life Science & Technology (Wuhan, China).

Techniques: In Vitro, Western Blot, Expressing